首页 文献索引 SCI期刊 AI助手
登录 注册
首页 正文

Molecular horticulture. 2025 Apr 1;5(1):18. doi: 10.1186/s43897-024-00139-7 Q110.02024

Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta)

染色体水平基因组组装有助于揭示醋栗猕猴桃（Actinidia arguta）花青素调节机制 翻译改进

Yukuo Li  1  2, Zhe Song  1  3, Xu Zhan  1  4, Xiaohan Li  1  3, Lingshuai Ye  1, Miaomiao Lin  1  2, Ran Wang  1, Hailei Huang  5, Jian Guo  6, Leiming Sun  1, Hong Gu  1, Jinyong Chen  1, Jinbao Fang  7, Xiujuan Qi  8  9

作者单位 +展开

作者单位

  • 1 National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China.
  • 2 Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang, 453500, China.
  • 3 School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China.
  • 4 College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471000, China.
  • 5 Shiyan Economic Crops Research Institute, Shiyan, 442099, China.
  • 6 College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, China.
  • 7 National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China. fangjinbao@caas.cn.
  • 8 National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China. qixiujuan@caas.cn.
  • 9 Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang, 453500, China. qixiujuan@caas.cn.

    • DOI: 10.1186/s43897-024-00139-7 PMID: 40165341

    摘要 Ai翻译

    Actinidia arguta is a newly emerged, commercially cultivated Actinidia species. A. arguta has a beautiful appearance and is rich in anthocyanin, and is thus highly welcomed by consumers. However, the mechanism of anthocyanin regulation in A. arguta remains unclear. In this study, we assembled the nearly complete genome of the first red A. arguta cultivar, 'Tianyuanhong', with an N50 of 21 Mb. Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A. arguta. Through verification of transient overexpression and stable transformation, RNA-seq analysis revealed a key bHLH transcription factor, AaBEE1, which negatively regulates anthocyanin biosynthesis. DAP-seq analysis combined with Y1H, EMSA, Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression. Overall, we assembled the genome of A. arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.

    Keywords: Actinidia arguta; Anthocyanin biosynthesis; Comparative genome; Gene expression; Genome assembly.

    Keywords：anthocyanin regulation; Actinidia arguta

    关键词：染色体级别的基因组装; 花青素调控; 羽叶木瓜

    Copyright © Molecular horticulture. 中文内容为AI机器翻译，仅供参考！

    相关内容

    期刊名：Molecular horticulture

    缩写：Mol Hortic

    ISSN：2730-9401

    e-ISSN：

    IF/分区：10.0/Q1

    文章目录 更多期刊信息

    全文链接
    官方链接
    PMC全文
    引文链接
    复制
    已复制！
    推荐内容
    Chromosome-level genome assembly assisting for dissecting mechanism of anthocyanin regulation in kiwifruit (Actinidia arguta)