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Analytical Validation and Greenness Assessment of HPLC-DAD Method for Methylxanthines Analysis

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In this study, a simple, and eco-friendly high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated for analyzing methylxanthines, including theobromine (TB), theophylline (TF), and caffeine (CF). This method de... ...