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ACS omega. 2025 Feb 8;10(6):6228-6233. doi: 10.1021/acsomega.4c11417 Q24.32025

Exonuclease-III Assisted Signal Cycle Integrating with Self-Priming Mediated Chain Extension for Sensitive and Reliable MicroRNA Detection

exo III介导的信号循环放大结合自引发链延伸对miRNA的灵敏检测 翻译改进

Chunmeng Li  1  2, Xiangjian Zheng  1, Shangshang Xie  1, Deyong Lin  1

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作者单位

  • 1 Department of Vascular Surgery, The Dingli Clinical Institute of Wenzhou Medical University (Wenzhou Central Hospital), Wenzhou City, Zhejiang Province 325000, China.
  • 2 Laboratory of Wenzhou Pan-Vascular Disease Management Center, Wenzhou City, Zhejiang Province 325000, China.

    • DOI: 10.1021/acsomega.4c11417 PMID: 39989823

    摘要 Ai翻译

    MicroRNA (miRNA) is pivotal in regulating pathological progression and may serve as a significant biomarker for early diagnosis, treatment, and management strategies for atherosclerosis. This study produced a self-priming amplification-accelerated CRISPR/Cas system-based method for the sensitive and selective detection of miRNA by merging Exo-III-assisted target recycling, self-priming-mediated chain extension, and the CRISPR/Cas12a system. The sensor comp... ...点击完成人机验证后继续浏览
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    期刊名：Acs omega

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    ISSN：2470-1343

    e-ISSN：2470-1343

    IF/分区：4.3/Q2

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    Exonuclease-III Assisted Signal Cycle Integrating with Self-Priming Mediated Chain Extension for Sensitive and Reliable MicroRNA Detection