Enhanced design of pCMViR-TSC plasmid vector for sustainably high cargo gene expression in mammalian cells
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The first-generation pCMViR-TSC, implemented through the promoter sandwich rule, yields 10- to 100-fold higher gene expression than the standard plasmid used with the CMV (cytomegalovirus) or CAG promoter. However, the vector's shortcomings limit its utility t... ...