Over the last decade, 3D bioprinting has gained increasing popularity, being a technique capable of producing well-defined tissue-like structures. One of its most groundbreaking features is the ability to create personalized therapies tailored to the specific demands of individual patients. However, challenges including the selection of materials and crosslinking strategies, still need to be addressed to enhance ink characteristics and develop robust biomaterials. Herein, the authors showcase the potential of overcoming these challenges, focusing on the use of versatile, fast, and selective thiol-ene click chemistry to formulate inks for 3D bioprinting. The exploration of natural polymers, specifically proteins and polysaccharides, will be discussed and highlighted, outlining the advantages and disadvantages of this approach. Leveraging advanced thiol-ene click chemistry and natural polymers in the development of 3D printable bioinks may face the current challenges and is envisioned to pave the way towards innovative and personalized biomaterials for biomedical applications.

Keywords: 3D bioprinting; Bioinks; Polysaccharides; Proteins; Regenerative medicine; Thiol-ene; Tissue engineering.

Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.

在过去十年里，3D生物打印技术越来越受欢迎，因为它能够生产出类似组织的结构。其最具开创性的特点之一是能够为个人患者的具体需求量身定制个性化治疗方案。然而，材料选择和交联策略等挑战仍然需要解决，以改进墨水特性并开发稳健的生物材料。在此文中，作者展示了克服这些挑战的潜力，并重点介绍了使用多功能、快速且具有选择性的硫醇-烯烃点击化学来配制用于3D生物打印的墨水的方法。将讨论和强调天然聚合物（特别是蛋白质和多糖）的应用及其优缺点。利用先进的硫醇-烯烃点击化学和天然聚合物开发可3D打印的生物墨水，有望克服当前面临的挑战，并为生物医学应用创新和个性化的生物材料铺平道路。

关键词：
3D生物打印；生物墨水；多糖；蛋白质；再生医学；硫醇-烯烃；组织工程。