Parasites & vectors. 2024 Oct 14;17(1):430. doi: 10.1186/s13071-024-06447-7 Q13.52025
Development of molecular diagnostic protocols for simultaneous identification of common bed bugs (Cimex lectularius) and tropical bed bugs (Cimex hemipterus)
常见床虫(Cimex lectularius)和热带床虫(Cimex hemipterus)分子诊断方法的建立及鉴定能力评估 翻译改进
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DOI: 10.1186/s13071-024-06447-7 PMID: 39402589
摘要 中英对照阅读
背景: 近期,两种床虫物种——普通床虫(Cimex lectularius Linnaeus, 1758)和热带床虫(Cimex hemipterus Fabricius, 1803)在同一地理区域的重新出现经常被报道。因此,由于这些物种在遗传和生理特性上的差异,快速识别这两种物种对于实施针对性捕捉陷阱和定制拟除虫菊酯抗性诊断至关重要。
方法: 为了开发分子诊断方法,根据特定种类的内转录间隔区2序列,使用种类特异性引物建立了多重PCR和环介导等温扩增(LAMP)的独特协议。这些方法被优化以实现这两种床虫物种的快速准确识别。
结果: 多重PCR和LAMP协议都能够同时鉴定这两种床虫物种,即使使用从死亡标本释放出的DNA也是如此。值得注意的是,LAMP简单直接的操作步骤和所需时间较少表明其在野外快速准确诊断床虫方面具有潜在的应用价值。这些方法的诊断准确性通过盲测进行了验证。
结论: 多重PCR和LAMP协议为快速准确地鉴定床虫种类奠定了基础,使其能够使用适当的陷阱并检测特定种类的拟除虫菊酯抗性突变。这种方法确保了针对每个床虫物种的独特特征进行有效的管理。
关键词: C. hemipterus; C. lectularius; 床虫; 环介导等温扩增; 分子识别; 多重PCR.
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