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F1000Research. 2023 Sep 8:12:660. doi: 10.12688/f1000research.133313.2 0.02025

Effect of green tea extract antioxidant on dentin shear bond strength and resin-tag penetration depth after non-vital bleaching

绿茶提取物抗氧化剂对非活髓漂白后树脂连接强度及树脂标记深度的影响 翻译改进

Darin Safinaz  1, Paramita Widyandari  1, Ratna Meidyawati  1, Citra Kusumasari  1, Dewa Ayu Nyoman Putri Artiningsih  1

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  • 1 Department of Conservative Dentistry, Faculty of Dentistry, University of Indonesia, Depok, West Java, 16424, Indonesia.
  • DOI: 10.12688/f1000research.133313.2 PMID: 39140086

    摘要 中英对照阅读

    Background: 35% Hydrogen peroxide (H 2O 2) as an active material for internal bleaching can produce free radicals that can affect resin tag penetration into the dentinal tubules. Application of 10% and 35% green tea (GT) extract as an antioxidant after 2 minutes are expected to remove free radical residues and increase dentin shear bond strength and resin tags penetration depth after non-vital bleaching. Methods: 30 extracted healthy human premolars were cut horizontally 2 mm from the Cemento Enamel Junction margin to the crown part, then cut in a mesio-distal direction into two parts. The specimens were divided into five groups: normal dentin, post bleaching dentin, delayed 2 weeks, 10% GT, and 35% GT group. Non-vital walking bleach with 35% H 2O 2 gel was done to all groups except control group. Soon after, 10% and 35% GT extract gel were applied on dentin for 2 minutes, then the specimens were rinsed-off with aquabidest for 2 minutes and dried. All specimens were etched and bonded with an etch-and-rinse adhesive system and filled with resin composite. The shear bond strength assessment was carried out using a Universal Testing Machine (UTM) with a cross-head speed of 0.5 mm/minute. Confocal laser scanning microscopy (CLSM) with a wavelength of 560 nm and a lens magnification of 40x was used to analyze the resin tag penetration. Data were analyzed by one way ANOVA and t-test. Results: There was a significant difference in resin tag penetration depth and shear bond strength between applying 10% and 35% GT extract (p < 0.05). The 35% GT extract group resulted in a significantly longer resin tag penetration than the 10% GT extract group. Conclusions: The application of 35% GT extract is more effective than 10% GT extract as an antioxidant for increasing the shear bond strength of composite resin after internal bleaching.

    Keywords: Tooth bleaching; antioxidant; green tea extract.

    Keywords:green tea extract; antioxidant; dentin shear bond strength

    背景:35%过氧化氢(H2O2)作为内部美白的活性材料,可以产生自由基,影响树脂在牙本质小管中的渗透。期望应用10%和35%绿茶(GT)提取物作为抗氧化剂,在使用过氧化氢后两分钟内去除自由基残留物,并增加非活髓美白后的牙本质剪切粘结强度及树脂标签的渗透深度。方法:从健康的人类前磨牙中切割出30颗牙齿,每颗牙齿在釉质牙骨质交界线2毫米处水平截断至冠部,然后沿近远中方向垂直切成两半。将样本分为五个组:正常牙本质、美白后牙本质、延迟两周、10% GT和35% GT组。除对照组外,所有组都进行了非活髓走动美白处理(使用35%过氧化氢凝胶)。随后,在各实验组的牙本质表面分别涂覆了10%和35%绿茶提取物凝胶两分钟,然后用二次蒸馏水冲洗2分钟后干燥。所有样本经过酸蚀处理并使用酸蚀-洗涤粘结系统与树脂复合材料进行粘结修复。通过万能试验机(UTM)以每分钟0.5毫米的速度评估剪切粘结强度,并利用共聚焦激光扫描显微镜(CLSM),采用波长为560纳米的40倍物镜来分析树脂标签的渗透情况。数据经单因素方差分析和t检验进行统计分析。结果:10%和35%绿茶提取物的应用在树脂标签渗透深度及剪切粘结强度方面存在显著差异(p 结论:应用35%绿茶提取物作为抗氧化剂比使用10%绿茶提取物更能提高非活髓美白后复合树脂的剪切粘结强度。

    关键词: 牙齿美白;抗氧化剂;绿茶提取物。

    关键词:绿茶提取物; 抗氧化剂; 牙本质剪切粘结强度

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    Effect of green tea extract antioxidant on dentin shear bond strength and resin-tag penetration depth after non-vital bleaching