Expression, purification and application of a recombinant, membrane permeating version of the light chain of botulinum toxin B
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Botulinum neurotoxins (BoNTs) are valuable tools to unveil molecular mechanisms of exocytosis in neuronal and non-neuronal cells due to their peptidase activity on exocytic isoforms of SNARE proteins. They are produced by Clostridia as single-chain polypeptide... ...
