Incautious design of shRNAs for stable overexpression of miRNAs could result in generation of undesired isomiRs
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shRNA-mediated strategy of miRNA overexpression based on RNA Polymerase III (Pol III) expression cassettes is widely used for miRNA functional studies. For some miRNAs, e.g., encoded in the genome as a part of a polycistronic miRNA cluster, it is most likely t... ...
