Strategies for single base gene editing in an immortalized human cell line by CRISPR/Cas9 technology
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The use of CRISPR/Cas9 system has rapidly grown in the last years. Here, the optimization of gene editing of a single-nucleotide polymorphism in a human non-malignant somatic cell line of thyrocytes (Nthy-Ori) was described highlighting strategies for overcomi... ...
