Optimization of the linear quantification range of an online trypsin digestion coupled liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform
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Tandem mass spectrometry (MS/MS)-based proteomic workflows with a bottom-up approach require enzymatic digestion of proteins to peptide analytes, usually by trypsin. Online coupling of trypsin digestion of proteins, using an immobilized enzyme reactor (IMER), ... ...
