Reducing metabolic burden in the PACEmid evolver system by remastering high-copy phagemid vectors
{{output}}
Orthogonal or non-cross-reacting transcription factors are used in synthetic biology as components of genetic circuits. Brödel et al. (2016) engineered 12 such cIλ transcription factor variants using a directed evolution 'PACEmid' system. The variants operat... ...
