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Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology. 2014 Jan;16(1):2078. doi: 10.1007/s11051-013-2078-3 Q32.12024

Single-walled carbon nanohorns decorated with semiconductor quantum dots to evaluate intracellular transport

用半导体量子点修饰的单壁碳纳米角用于评价细胞内运输 翻译改进

Kristen A Zimmermann  1, David L Inglefield Jr  2, Jianfei Zhang  3, Harry C Dorn  4, Timothy E Long  3, Christopher G Rylander  1  5, M Nichole Rylander  1  5

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作者单位

  • 1 School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, ICTAS Building, Stanger Street, Blacksburg, VA 24061, USA.
  • 2 Department of Chemistry, Virginia Polytechnic Institute and State University, ICTAS II Building, Washington Street, Blacksburg, VA 24061, USA.
  • 3 Department of Chemistry, Virginia Polytechnic Institute and State University, Hahn Hall South Building, Blacksburg, VA 24061, USA.
  • 4 Department of Chemistry, Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, VA 24016, USA.
  • 5 Department of Mechanical Engineering, Virginia Polytechnic Institute and State University, ICTAS Building, Stanger Street, Blacksburg, VA 24061, USA.
  • DOI: 10.1007/s11051-013-2078-3 PMID: 36935903

    摘要 Ai翻译

    Single-walled carbon nanohorns (SWNHs) have great potential to enhance thermal and chemotherapeutic drug efficiencies for cancer therapies. Despite their diverse capabilities, minimal research has been conducted so far to study nanoparticle intracellular transport, which is an important step in designing efficient therapies. SWNHs, like many other carbon nanomaterials, do not have inherent fluorescence properties making intracellular transport information difficult to obtain. The goals of this project were to (1) develop a simple reaction scheme to decorate the exohedral surface of SWNHs with fluorescent quantum dots (QDs) and improve conjugate stability, and (2) evaluate SWNH-QD conjugate cellular uptake kinetics and localization in various cancer cell lines of differing origins and morphologies. In this study, SWNHs were conjugated to CdSe/ZnS core/shell QDs using a unique approach to carbodiimide chemistry. Transmission electron microscopy and electron dispersive spectroscopy verified the conjugation of SWNHs and QDs. Cellular uptake kinetics and efficiency were characterized in three malignant cell lines: U-87 MG (glioblastoma), MDA-MB-231 (breast cancer), and AY-27 (bladder transitional cell carcinoma) using flow cytometry. Cellular distribution was verified by confocal microscopy, and cytotoxicity was also evaluated using an alamarBlue assay. Results indicate that cellular uptake kinetics and efficiency are highly dependent on cell type, highlighting the significance of studying nanoparticle transport at the cellular level. Nanoparticle intracellular transport investigations may provide information to optimize treatment parameters (e.g., SWNH concentration, treatment time, etc.) depending on tumor etiology.

    Keywords: Cancer; Cellular distribution; Nanobiotechnology; Quantum dot (QD); Single-walled carbon nanohorn (SWNH); Uptake kinetics.

    Keywords:single-walled carbon nanohorns; semiconductor quantum dots; intracellular transport

    Copyright © Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology. 中文内容为AI机器翻译,仅供参考!

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    期刊名:Journal of nanoparticle research

    缩写:J NANOPART RES

    ISSN:1388-0764

    e-ISSN:1572-896X

    IF/分区:2.1/Q3

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