Nanofibers comprising polystyrene and zinc acetate for extraction of salivary histidine prior to its determination by HPLC-DAD
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A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC-DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine wit... ...