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Biomolecular NMR assignments. 2022 Apr;16(1):113-119. doi: 10.1007/s12104-022-10068-5 Q40.82024

Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Hα-detected experiments

基于Hα检测实验的Olduvai蛋白结构域CON1无序状态下的NMR主链共振峰指派 翻译改进

Natasia Paukovich  1, Morkos A Henen  1  2, Alya Hussain  1, Aaron Issaian  1, James M Sikela  1, Kirk C Hansen  1, Beat Vögeli  3

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作者单位

  • 1 Department of Biochemistry & Molecular Genetics, School of Medicine, University of Colorado, 12801 E. 17th Avenue, Aurora, CO, 80045, USA.
  • 2 Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
  • 3 Department of Biochemistry & Molecular Genetics, School of Medicine, University of Colorado, 12801 E. 17th Avenue, Aurora, CO, 80045, USA. beat.vogeli@cuanschutz.edu.
  • DOI: 10.1007/s12104-022-10068-5 PMID: 35098449

    摘要 Ai翻译

    Olduvai protein domains, encoded by the NBPF gene family, are responsible for the largest increase in copy number of any protein-coding region in the human genome. This has spawned various genetics studies which have linked these domains to human brain development and divergence from our primate ancestors, as well as currently relevant cognitive diseases such as schizophrenia and autism spectrum disorder (ASD). There are six separate Olduvai domains which together form the majority of the various protein products of the NBPF genes. The six domains include three conserved domains (CON1-3), and three human-lineage-specific domains (HLS1-3) which occur in triplet. Here, we present the solution nuclear magnetic resonance backbone assignments for the CON1 domain, which has been linked to the severity of ASD. The data confirm that CON1 is an intrinsically disordered protein (IDP). Additionally, we use innovative Hα-detected experiments which allow us to not only assign the Hα atoms and N atoms of proline residues, but also to assign residues where HN-experiments suffered from peak overlap or broadening.

    Keywords: Autism; Backbone chemical shift assignment; DUF1220; IDP; Olduvai domain.

    Keywords:solution nmr; backbone assignments; disordered proteins; olduvai protein; con1 domain

    Copyright © Biomolecular NMR assignments. 中文内容为AI机器翻译,仅供参考!

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    期刊名:Biomolecular nmr assignments

    缩写:BIOMOL NMR ASSIGN

    ISSN:1874-2718

    e-ISSN:1874-270X

    IF/分区:0.8/Q4

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    Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Hα-detected experiments