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Comparative Evaluation of Natural Killer Cell-mediated Cell Killing Assay Based on the Leakage of an Endogenous Enzyme or a Pre-loaded Fluorophore

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A cell-killing ability of natural killer (NK) cells has been evaluated by the leakage of marker molecules from target cells. Lactate dehydrogenase (LDH) and calcein are two major non-radioisotope markers used for the killing assay. The spontaneous death of NK ... ...