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Poly-adenine-Coupled LAMP Barcoding to Detect Apple Scar Skin Viroid

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Apple Scar Skin Viroid (ASSVd), a nonprotein coding, circular RNA pathogen is relatively difficult to detect by immunoassay. We report here a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to improve selectivity for diagn... ...