A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling techniques. Besides, nano-sized particles could be observed and located by dark field microscopes. Therefore, comparing with the commercial Raman spectrometers, much more researches based on Raman spectroscopy could be carried out on this integrated Raman platform, especially in the fields of analyzing biological tissues and subwavelength samples.
The Review of scientific instruments. 2014 May;85(5):056109. doi: 10.1063/1.4878715 Q31.72025
Note: Raman microspectroscopy integrated with fluorescence and dark field imaging
注：拉曼显微光谱与荧光及暗场成像的联用技术 翻译改进
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DOI: 10.1063/1.4878715 PMID: 24880434
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