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Visualization of the network of primo vessels and primo nodes above the pia mater of the brain and spine of rats by using Alcian blue

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By spraying and injecting Alcian blue into the lateral ventricle, we were able to visualize the network of the nerve primo vascular system above the pia mater of the brain and spine of rats. Staining these novel structures above the pia mater with 4',6-diamidi... ...