Structure, dynamics, and ionization equilibria of the tyrosine residues in Bacillus circulans xylanase
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We have developed NMR spectroscopic methods to investigate the tyrosines within Bacillus circulans xylanase (BcX). Four slowly exchanging buried tyrosine hydroxyl protons with chemical shifts between 7.5 and 12.5 ppm were found using a long-range (13)C-HSQC ex... ...