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Insect biochemistry and molecular biology. 2008 Oct;38(10):963-8. doi: 10.1016/j.ibmb.2008.07.008 Q13.72025

Transgene expression and silencing in a tick cell line: A model system for functional tick genomics

一种硬蜱细胞系的转基因表达与沉默:功能基因组研究模型系统 翻译改进

Timothy J Kurtti  1, Joshua T Mattila, Michael J Herron, Roderick F Felsheim, Gerald D Baldridge, Nicole Y Burkhardt, Bruce R Blazar, Perry B Hackett, Jason M Meyer, Ulrike G Munderloh

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  • 1 Department of Entomology, University of Minnesota, Saint Paul, MN 55108, USA. kurtt001@umn.edu
  • DOI: 10.1016/j.ibmb.2008.07.008 PMID: 18722527

    摘要 Ai翻译

    The genome project of the black legged tick, Ixodes scapularis, provides sequence data for testing gene function and regulation in this important pathogen vector. We tested Sleeping Beauty (SB), a Tc1/mariner group transposable element, and cationic lipid-based transfection reagents for delivery and genomic integration of transgenes into I. scapularis cell line ISE6. Plasmid DNA and dsRNA were effectively transfected into ISE6 cells and they were successfu... ...点击完成人机验证后继续浏览
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    期刊名:Insect biochemistry and molecular biology

    缩写:INSECT BIOCHEM MOLEC

    ISSN:0965-1748

    e-ISSN:1879-0240

    IF/分区:3.7/Q1

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    Transgene expression and silencing in a tick cell line: A model system for functional tick genomics