In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1-2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27-34% decrease in cell number but stimulated an 800-1200% increase in the production of prostaglandin E(2), an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.
Journal of biotechnology. 2008 Feb 29;133(4):490-6. doi: 10.1016/j.jbiotec.2007.11.010 Q23.92025
Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds
一种用于顺应性组织工程支架的动态流体灌注生物反应器的设计与验证 翻译改进
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DOI: 10.1016/j.jbiotec.2007.11.010 PMID: 18221813
摘要 Ai翻译
Keywords：tissue engineering scaffolds
关键词：组织工程支架
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