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Validation of sensitive human leukocyte antigen-sequence-specific primer and probe typing in forensic DNA examination

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Validation studies were carried out with the commercially available HLA typing kit using a PCR-SPP (sequence-specific primer and probe) technique. This technique has made it possible to type class I (HLA-A and -B) and class II (HLA-DRB1 and -DQB1) alleles at l... ...