Quantification of ofloxacin in biological fluids by high-performance liquid chromatography
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A simple HPLC assay was evaluated for analysis of ofloxacin in biological fluids. Mobile phase contained methanol (40%) and phosphate buffer (pH 3.5, 60 mmol/l) and a common C18 column was used. Detection was set at 280 nm (UV). Biological fluids (50 microlite... ...