The 49 kDa penicillin-binding protein (PBP) of Mycobacterium smegmatis catalyses the hydrolysis of the peptide or S-ester bond of carbonyl donors R1-CONH-CHR2-COX-CHR2-COO- (where X is NH or S). In the presence of a suitable amino acceptor, the reaction partitions between the transpeptidation and hydrolysis pathways, with the amino acceptor, behaving as a simple alternative nucleophile at the level of the acyl-enzyme. By virtue of its N-terminal sequence similarity, the 49 kDa PBP represents one of the class of monofunctional low-molecular-mass PBPs. An immunologically related protein of M(r) 52,000 is present in M. tuberculosis. The 49 kDa PBP is sensitive towards amoxycillin, imipenem, flomoxef and cefoxitin.
The Biochemical journal. 1996 Nov 15;320 ( Pt 1)(Pt 1):197-200. doi: 10.1042/bj3200197 Q24.32025
Biochemical characterization of the 49 kDa penicillin-binding protein of Mycobacterium smegmatis
龟分枝杆菌49千道尔顿肽聚糖交联酶的生化性质研究 翻译改进
作者单位 +展开
作者单位
DOI: 10.1042/bj3200197 PMID: 8947487
摘要 Ai翻译
Keywords：penicillin-binding protein
关键词：青霉素结合蛋白
相关内容
-
Anti-Metatype Antibody Screening, Sandwich Immunoassay Development, and Structural Insights for β-Lactams Based on Penicillin Binding Protein
基于青霉素结合蛋白的抗代谢类型抗体筛选，夹心免疫测定开发以及β-内酰胺类药物的结构见解
Molecules (Basel, Switzerland). 2021 Sep 13;26(18):5569.
-
The structures of penicillin-binding protein 4 (PBP4) and PBP5 from Enterococci provide structural insights into β-lactam resistance
肠球菌青霉素结合蛋白4(PBP4)和PBP5的结构为β-内酰胺耐药性提供结构见解
The Journal of biological chemistry. 2018 Nov 30;293(48):18574-18584.
-
Biocatalytic Tetrapeptide Macrocyclization by Cryptic Penicillin-binding Protein-type Thioesterases
隐秘青霉素结合蛋白类型的硫酯酶的生物催化四肽宏环化
bioRxiv : the preprint server for biology. 2024 Nov 16:2024.11.16.623930.
-
A Review on Five and Six-Membered Heterocyclic Compounds Targeting the Penicillin-Binding Protein 2 (PBP2A) of Methicillin-Resistant Staphylococcus aureus (MRSA)
关于针对耐甲氧西林金黄色葡萄球菌青霉素结合蛋白2(PBP2A)的五元和六元杂环化合物的研究进展综述
Molecules (Basel, Switzerland). 2023 Oct 10;28(20):7008.
-
Unraveling the mechanism of ceftaroline-induced allosteric regulation in penicillin-binding protein 2a: insights for novel antibiotic development against methicillin-resistant Staphylococcus aureus
揭秘达托霉素通过结合MprF蛋白抑制耐甲氧西林金黄色葡萄球菌生物被膜形成的机制：为新型抗生物被膜药物开发提供见解
Antimicrobial agents and chemotherapy. 2023 Dec 14;67(12):e0089523.
-
Binding of (5S)-penicilloic acid to penicillin binding protein 3
(5S)青霉醛酸与青霉素结合蛋白3的结合研究
ACS chemical biology. 2013 Oct 18;8(10):2112-6.
-
Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis
酿酒酵母PrsA肽基-脯氨酰-tRNA合成酶对枯草芽孢杆菌结合蛋白折叠的影响
Molecular microbiology. 2010 Jul 1;77(1):108-27.
-
Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography
改良型金黄色葡萄球菌青霉素结合蛋白2a的构建及金属离子亲和介质层析法纯化
Journal of bacteriology. 1994 Mar;176(5):1539-41.
