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Detection of antibodies to Cowdria ruminantium in the serum of domestic ruminants by indirect ELISA

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A solid phase enzyme immunoassay for the detection of antibodies to Cowdria ruminantium in the serum of domestic ruminants was developed by using microorganisms cultivated on bovine umbilical endothelial cells as antigen. When the culture showed 90% lysis, the... ...