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Separation of two subpopulations of old human fibroblasts by mitochondria (rhodamine 123) fluorescence

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The mitochondria-binding fluorescent dye rhodamine 123 (R123) was used to sort two subpopulations of cells from mass cultures of old human fibroblasts (HF). Both subpopulations showed unusual dye retention kinetics when compared to young HF. Additionally, elec... ...