[A simple thin-layer chromatographic assay of urocanic acid in the stratum corneum for evaluation of skin histidase activity (author's transl)]
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A simple and rapid urocanic acid estimation by mean of TLC as an indirect assay for skin histidase activity is described. Very small amounts of stratum corneum (about 1 mg) are eluated in concentrated NH3, 5 mul of the eluate were chromatographied and stained ... ...