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Replication of M-13 DNA in plamolysed Escherichia coli cells. Formation of fully synthetic duplex DNA

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The replication of the double-stranded replicative-form DNA of bacteriophage M-13 was studied in a cellular system in vitro prepared by plasmolysis of M-13-am5-infected Escherichia coli cells. Newly synthesized DNA was density-labelled with bromodeoxyuridine t... ...